| 講演要旨： |
Heat transfer plays a crucial role in many biomedical applications in cryobiology (biopreservation and cryosurgery) and hyperthermic biology (thermal therapies). In these applications, thermal excursions are used to selectively preserve or destroy cells and tissues. Biopreservation is an enabling technology to many biomedical fields including cell and tissue banking, cell therapeutics, tissue engineering, organ transplantation and assisted reproductive technologies. Thermal therapies including cryosurgery are increasingly important in all surgical sub-specialties for minimally invasive thermal destruction of tissues for cancer and cardiovascular disease treatment. In this talk work predominantly from our lab will be reviewed focusing on cellular and molecular phenomena that are important in defining outcomes of both cryobiological and hyperthermic biomedical applications. During these applications microscale cellular phenomena depend on the heat transfer process, such as cellular dehydration, intracellular ice formation, and membrane hyperpermeability and blebbing which are all mechanistically linked to viability change. Molecular and nanoscale events include lipid oxidation, phase change and segregation as well as protein denaturation which are mechanistically linked to viability changes. Function can also be affected as for example both heating and freezing changes to collagen can change biomechanical properties, especially in some cardiovascular tissues. In addition, gene regulated events (inflammation and apoptosis), often in response to thermally triggered molecular changes, have recently been shown to increase thermal destruction of cells. Finally, recent work will be reviewed with iron oxide and gold nanoparticles showing their dramatic potential to both enhance and control thermal therapy outcomes through adjuvant (drug) delivery, tomographic visualization and inductive heating within the body. |
